Fluorescent Biosensors in the Detection of Mycotoxins: Working Principles and Applications

Abstract

Due to their superior sensitivity and specificity, fluorescent biosensors have become highly effective tools for detecting mycotoxins. This thesis delves into the fundamental principles, material choices, and technological advancements that underpin these biosensors. Special attention is given to the role of aptamer technology. It involves synthetic nucleic acids which is known for the exceptional specificity and binding affinity. The aptamers significantly enhance biosensor performance by offering greater design versatility and more robust target recognition than conventional antibodies. Incorporating quantum dots and fluorescent dyes as signal labels further enhances the optical capabilities of these biosensors, enabling precise multicolour imaging and the ability to conduct multiplexed detection. This study underscores the essential function of fluorescent biosensors in tackling food safety challenges, particularly those bring about by mycotoxins. These sensors provide rapid and accurate detection methods for analysing complex sample matrices. The research findings presented in this work offer valuable insights into optimizing biosensor technology. This work would contribute to the advancement in food safety, environmental monitoring, and medical diagnostics. By deepening the understanding of these technologies, this work sets the stage for future innovations to protect public health further and enhance safety measures.