Immunoevaluation of hydrophobic modified polyethyleneimine as cationic polymer adjuvant
DOI:
https://doi.org/10.62051/19x1e293Keywords:
Polyethyleneimine, Adjuvant, Nanoparticle, Immunoevaluation.Abstract
Polyethyleneimine (PEI) is a cationic polymer that has excellent gene transfection effects as a gene transfection reagent. Its excellent proton sponge effect has attracted much attention in the field of vaccine adjuvants. However, its high positive charge density leads to high toxicity, which limits its application. In this paper, polyethyleneimine modified by stearyl chloride was used to reduce toxicity to obtain SA-PEI. The partical size of composite particles formed by SA-PEI adjuvant and ovalbumin (OVA) were smaller than 200 nm. In vivo experiments of Balb/c mice experiments showed that, compared with unmodified PEI, SA-PEI could significantly enhance the specific immune response to OVA and may promote the endocytosis of macrophages. This study clearly provides a promising cationic polymer adjuvant for the vaccine field.
Downloads
References
S.G. Reed, M.T. Orr, C.B. Fox, Key Roles of Adjuvants in Modern Vaccines, Nature Medicine 19(12) (2013) 1597-1608.
R.L. Coffman, A. Sher, R.A. Seder, Vaccine Adjuvants: Putting Innate Immunity to Work, Immunity 33(4) (2010) 492-503.
S. Shi, H. Zhu, X. Xia, Z. Liang, X. Ma, B. Sun, Vaccine Adjuvants: Understanding the Structure and Mechanism of Adjuvanticity, Vaccine 37(24) (2019) 3167-3178.
A.S. Mckee, M.W. Munks, P. Marrack, Towards an Understanding of the Adjuvant Action of Aluminium, Nature Reviews Immunology 9(4) (2009) 287.
S. Kommareddy, M. Singh, D.T. O'Hagan, MF59: A Safe and Potent Adjuvant for Human Use - ScienceDirect, Immunopotentiators in Modern Vaccines (Second Edition) (2017) 249-263.
D.S. Tretiakova, E.L. Vodovozova, Liposomes as Adjuvants and Vaccine Delivery Systems, Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology 16(1) (2022) 1-20.
W.G.J. Degen, Schijns V.E.J.C., Host-Derived Cytokines and Chemokines as Vaccine Adjuvants, Immunopotentiators in Modern Vaccines (2017) 65-84.
M. Garinot, F. Piras-Douce, P. Probeck, V. Chambon, J. Haensler, A potent Novel Vaccine Adjuvant Based on Straight Polyacrylate, International Journal of Pharmaceutics X 2 (2020) 100054.
D.J. Chand, R.B. Magiri, H.L. Wilson, G.K. Mutwiri, Polyphosphazenes as Adjuvants for Animal Vaccines and Other Medical Applications, Frontiers in Bioengineering and Biotechnology 9 (2021) 625482.
D. Li, Y. Ping, F. Xu, H. Yu, H. Pan, H. Huang, Q. Wang, G. Tang, J. Li, Construction of a Star-Shaped Copolymer as a Vector for FGF Receptor-Mediated Gene Delivery in vitro and in vivo, Biomacromolecules 11(9) (2010) 2221-2229.
Z. Chen, Z. Lv, Y. Sun, Z. Chi, G. Qing, Recent Advancements in Polyethyleneimine-based Materials and their Biomedical, Biotechnology, and Biomaterial Applications, Journal of Materials Chemistry B 8(15) (2020) 2951-2973.
X. Wang, D. Niu, C.-Z. Hu, P. Li, Polyethyleneimine-Based Nanocarriers for Gene Delivery, Current Pharmaceutical Design 21(42) (2015) 6140-6156.
N.C. Sheppard, S.A. Brinckmann, K.H. Gartlan, M. Puthia, C. Svanborg, G. Krashias, S.C. Eisenbarth, R.A. Flavell, Q.J. Sattentau, F. Wegmann, Polyethyleneimine is a Potent Systemic Adjuvant for Glycoprotein Antigens, International Immunology 26(10) (2014) 531-538.
M.L. Forrest, G.E. Meister, J.T. Koerber, D.W. Pack, Partial Acetylation of Polyethylenimine Enhances In vitro Gene Delivery, Pharmaceutical Research (Dordrecht), 21 (2004) 365-371.
Downloads
Published
Conference Proceedings Volume
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
