Progress in The Application of CRISPR-Cas9 Combined With Microbial Engineering

Xindan Xing

doi:10.62051/96r9n194

Authors

Xindan Xing

DOI:

https://doi.org/10.62051/96r9n194

Keywords:

CRISPR - Cas9; microbial engineering; E. coli; algae.

Abstract

CRISPR - Cas9 as the third generation of gene editing techniques, widely exists in prokaryotes, and has been widely applied to clinical and pharmaceutical fields. This article reviews the discovery, immune defense process and mechanism of CRISPR-Cas9 and summarizes its application in microbial cells. Advances include achieving gene editing in E. coli, improving defense against phages, and stabilizing chemical production; It is used to modify the characteristics of lactic acid bacteria and precise genome editing in algae; In terms of viruses, it can be used to treat HIV-1 infection, hemoglobinopathy, etc. Research gaps include less research on the effect of optimal length and selective markers of donor DNA homologous arms on efficiency in bacterial gene editing and insufficient evaluation of potential off-target effects and long-term effects of CRISPR/Cas9 systems in algae. This paper mainly analyzed the application of CRISPR-Cas9 in bacteria, lactic acid bacteria, algae, and viruses, and obtained corresponding research results. It provides a theoretical basis and a new idea for the research in the field of microbiology, but there are still some problems to be solved, and future research can focus on optimizing the system, reducing the off-target rate, and improving the editing efficiency.

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References

[1] Deng Ming & Ding Junmei. (2022). Application of CRISPR/Cas9 gene editing technology in microbial cells. Chinese Journal of Applied & Environmental Biology (03), 787-795. (In Chinese) doi: 10.19675/j.cnki.1006-687x.2021.01051.

[2] Chen, S., Nai, Z., Qin, Z., Li, G., He, X., Wang, W., ... & Jiang, X. (2023). The extracellular polysaccharide inhibits porcine epidemic diarrhea virus with extract and gene editing Lacticaseibacillus. Microbial Cell Factories, 22(1), 225.

[3] Chai, R., Guo, J., Geng, Y., Huang, S., Wang, H., Yao, X., ... & Qiu, L. (2024). The Influence of Homologous Arm Length on Homologous Recombination Gene Editing Efficiency Mediated by SSB/CRISPR-Cas9 in Escherichia coli. Microorganisms, 12(6), 1102.

[4] Nymark, M., Sharma, A. K., Sparstad, T., Bones, A. M., & Winge, P. (2016). A CRISPR/Cas9 system adapted for gene editing in marine algae. Scientific reports, 6(1), 24951.

[5] Wei, J., & Li, Y. (2023). CRISPR-based gene editing technology and its application in microbial engineering. Engineering Microbiology, 3(4), 100101.

[6] Jiang, F., & Doudna, J. A. (2017). CRISPR–Cas9 structures and mechanisms. Annual review of biophysics, 46(1), 505-529.

[7] Redman, M., King, A., Watson, C., & King, D. (2016). What is CRISPR/Cas9. Archives of Disease in Childhood-Education and Practice, 101(4), 213-215.

[8] Jiang WY, Bikard D, Cox D, Zhang F, Marraffini LA. RNA-guided editing of bacterial genomes using CRISPR-Cas’s systems [J]. Nat Biotechnol, 2013, 31: 233-239

[9] Li, Q., Sun, B., Chen, J., Zhang, Y., Jiang, Y. U., & Yang, S. (2021). A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochimica et Biophysica Sinica, 53(5), 620-627.

[10] Liu, L., Zhao, D., Ye, L., Zhan, T., **ong, B., Hu, M., ... & Zhang, X. (2020). A programmable CRISPR/Cas9-based phage defense system for Escherichia coli BL21 (DE3). Microbial cell factories, 19, 1-9.

[11] Su, B., Song, D., & Zhu, H. (2020). Homology-dependent recombination of large synthetic pathways into E. coli genome via λ-Red and CRISPR/Cas9 dependent selection methodology. Microbial Cell Factories, 19, 1-11.

[12] Cao, Z., Ma, Y., Jia, B., & Yuan, Y. J. (2022). Mobile CRISPR-Cas9 based anti-phage system in E. coli. Frontiers of Chemical Science and Engineering, 16(8), 1281-1289.

[13] PIJKEREN J-P V, BARRANGOU R. Genome editing of Food-Grade lactobacilli to develop therapeutic probiotics[M]. Bugs as Drugs, 2018:389-408.

[14] Börner, R. A., Kandasamy, V., Axelsen, A. M., Nielsen, A. T., & Bosma, E. F. (2019). Genome editing of lactic acid bacteria: opportunities for food, feed, pharma and biotech. FEMS microbiology letters, 366(1), fny291.

[15] Mu, Y., Zhang, C., Li, T., **, F. J., Sung, Y. J., Oh, H. M., ... & **, L. (2022). Development and applications of CRISPR/Cas9-based genome editing in Lactobacillus. International Journal of Molecular Sciences, 23(21), 12852.

[16] Wu, Z. Y., Huang, Y. T., Chao, W. C., Ho, S. P., Cheng, J. F., & Liu, P. Y. (2019). Reversal of carbapenem-resistance in Shewanella algae by CRISPR/Cas9 genome editing. Journal of advanced research, 18, 61-69.

[17] Badis, Y., Scornet, D., Harada, M., Caillard, C., Godfroy, O., Raphalen, M., ... & Cock, J. M. (2021). Targeted CRISPR‐Cas9‐based gene knockouts in the model brown alga Ectocarpus. New Phytologist, 231(5), 2077-2091.

[18] Naduthodi, M. I. S., Barbosa, M. J., & van der Oost, J. (2018). Progress of CRISPR‐Cas based genome editing in photosynthetic microbes. Biotechnology journal, 13(9), 1700591.

[19] Zaidi, S. S. E. A., Tashkandi, M., Mansoor, S., & Mahfouz, M. M. (2016). Engineering plant immunity: using CRISPR/Cas9 to generate virus resistance. Frontiers in plant science, 7, 1673.

[20] Khoshandam, M., Soltaninejad, H., Mousazadeh, M., Hamidieh, A. A., & Hosseinkhani, S. (2024). Clinical applications of the CRISPR/Cas9 genome-editing system: Delivery options and challenges in precision medicine. Genes & Diseases, 11(1), 268-282.