Research on Rapid Detection of Salmonella and Listeria monocytogenes Using Colloidal Gold Immunoassay and PCR Methods

Guoqiang Fu; Zhesheng Ning; Enxu Wang

doi:10.62051/ijphmr.v2n2.06

Authors

Guoqiang Fu
Zhesheng Ning
Enxu Wang

DOI:

https://doi.org/10.62051/ijphmr.v2n2.06

Keywords:

Salmonella, Listeria monocytogenes, Colloidal gold immunoassay strips, PCR (probe method) rapid detection kits, Detection methods, Accuracy

Abstract

This study used colloidal gold immunoassay strips and PCR (probe method) kit methods to detect Salmonella and Listeria monocytogenes. The accuracy and precision of the colloidal gold immunoassay strips and PCR (probe method) kit methods were validated using proficiency testing blind samples, and the consistency with the current national gold standard method was compared. The results of the two rapid detection methods were consistent with the results of the national standard method. This paper also analyzed the detection time and cost of the two rapid detection methods and the national standard method. The PCR (probe method) kit method takes less time for detection and can be used for high-throughput detection, with high sensitivity and low interference. It requires high equipment and personnel capabilities. The colloidal gold immunoassay strips method has the lowest detection cost, but its sensitivity is lower than that of the PCR method. It is easy to operate and requires low personnel and equipment requirements. Laboratories can choose the appropriate method based on their actual needs.

References

[1] LI S, HAN D P, PENG Y, et al. Research progress of food safety rapid detection technology [J]. Journal of Food Safety & Quality, 2019, 10(17):5575-5581

[2] MACHODO I, GARRIDO V, HERNANDAZ LI, et al. Rapid and specific detection of Salmonella infections using chemically modified nucleic acid probes [J]. Anal Chim Acta, 2018, 12(27):1-10.

[3] WHO/FAO. Risk assessment of Listeria monocytogenes in readyto-eat foods: Interpretative Summary [C]. Microbiological risk assessment series, 2004

[4] THOMAS J, GOVENDER N, MCCARTHY K M, et al. Outbreak of listeriosis in South Africa associated with processed meat [J]. New England Journal of Medicine, 2020, 382(7): 632-643.

[5] ALLENDE A, BARBUDDHE S B, DEVLEESSCHAUWER B, et al. Listeria monocytogenes in ready-to-eat (RTE) foods: attribution, characterization and monitoring: Meeting report [R]. 2022, FAO/ WHO.

[6] LI W W, GUO Y C, LIU Z T, et al. Analysis of foodborne disease outbreaks in China Mainland in 2016 [J]. Chinese Journal of Food Hygiene, 2022, 34(1):86-91.

[7] LI H Q, JIA H Y, ZHAO S, et al. Analysis of foodborne disease outbreaks in Chinese Mainland in 2021 [J]. Chinese Journal of Food Hygiene, 2022, 34(4):816-821.

[8] FITZGERALD C, COLLINS M, VAN DUYNE S, et al. Multiplex, bead-based suspension array for molecular determination of common Salmonella serogroups [J]. Journal of Clinical Microbiology, 2007, 45(10):3323-3334.

[9] KIM K, KIM D, SUN J S, et al. Comparative evaluation of the murine immune responses to Salmonella enterica serovars Enteritidis, Gallinarum and Typhimurium infection [J]. Korean Journal of Veterinary Research, 2013, 53(2):95-101.

[10] MALORNY B, HUEHN S, DIECKMANN R, et al. Polymerase chain reaction for the rapid detection and serovar identification of Salmonella in food and feeding stuff [J]. Food Analytical Methods, 2009, 2(2):81-95.

[11] National Health and Family Planning Commission, National Food and Drug Administration. Food safety national standards, Food microbiological analysis, Salmonellatesting: GB 4789.4— 2016 [S]. Beijing: Standards Press of China, 2016.

[12] National Health and Family Planning Commission, National Food and Drug Administration. National food safety standards - Food microbiological examination-Examination of Listeria monocytogenes: GB 4789.30—2016 [S] Beijing: Standards Press of China, 2017.