Calcium Phosphate/Polyacrylamide/Calcium Alginate Hybrid Hydrogel Membranes for the Controlled Release of Bovine Serum Albumin

Xianmei Ma; Siyu Wang; Kongyin Zhao; Xiaoyin Wang

doi:10.62051/ijmsts.v5n3.01

Authors

Xianmei Ma
Siyu Wang
Kongyin Zhao
Xiaoyin Wang

DOI:

https://doi.org/10.62051/ijmsts.v5n3.01

Keywords:

Polyacrylamide/Calcium alginate, Hydrogel membrane, Diammonium hydrogen phosphate, BSA, Controlled release

Abstract

A polyacrylamide/calcium alginate (PAM/CaAlg) hybrid hydrogel membrane was prepared by UV-initiated free-radical polymerization and subsequent Ca2+ ionic crosslinking. Then the PAM/CaAlg membrane was further treated with diammonium hydrogen phosphate (DHP) solutions to induce calcium phosphate. The resulted calcium phosphate/polyacrylamide/calcium alginate (CP/PAM/CaAlg) hybrid hydrogel membrane was immersed in bovine serum albumin (BSA) aqueous solution for sufficient adsorption. BSA was used as a model protein to investigate the controlled-release behavior of the hybrid hydrogel membranes. The effects of acrylamide/sodium alginate mass ratio, DHP concentration and saline treatment on the swelling behavior and BSA release performance were studied. The morphology of the hydrogel membranes was characterized by scanning electron microscopy (SEM). The results showed that the CP/PAM/CaAlg hybrid hydrogel membranes exhibited a porous structure, which facilitated BSA loading. Meanwhile, the calcium phosphate phase and the hybrid polymer network helped regulate the diffusion pathway of BSA, thereby improving the sustained-release performance. The swelling behavior of the membranes could be regulated by changing the polymer composition and the concentration of DHP. Compared with saline-treated membranes, the phosphate-treated membranes showed better sustained-release performance for BSA in Tris-HCl buffer. The prepared CP/PAM/CaAlg hybrid hydrogel membranes have potential application as protein drug controlled release.

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